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KMID : 1123920120260020175
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Korean Journal of Oriental Physiology and Pathology
2012 Volume.26 No. 2 p.175 ~ p.180
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Anti-inflammatory Effects of New Herbal Formula Cheongyeoungkeumyeum
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Kim Seung-Tae
Lee Boo-Kyun
Woo Won-Hong
Kim Hyung-Woo
Lee Young-Cheol
Lee Jung-Bok
Lee Jang-Cheon
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Abstract
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The purpose of this study is to verify the anti-inflammatory effectiveness of Cheongyeoungkeumyeum(CYK) including seven herbal medicines of that major effectiveness is to clear heat, to relieve fire toxicity and to clear damp-heat. To evaluate anti-inflammatory effectiveness of CYK, we measured the production of reactive oxygen species(ROS), nitric oxide(NO) cyclooxygenase-2(COX-2) and in TNF-α LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF-α were detected by enzyme immuno assay(EIA) and enzyme-linked immunosorbent assay(ELISA). As a result, we found that CYK suppressed LPS-induced ROS and NO production in a dose-dependent manner. Also CYK significantly inhibited LPS-induced COX-2 activity and the release of TNF-α. These results indicate that the CYK may have an anti-inflammatory agent for the treatment of various inflammatory disease.
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KEYWORD
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Cheongyeoungkeumyeum(CYK), ROS, NO, COX-2, TNF-, LPS, Raw 264.7
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